Journal: Cell Reports Medicine

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state

doi: 10.1016/j.xcrm.2024.101869

Figure Lengend Snippet: Miltefosine enhances CAR-T cell efficacy against solid tumors in vivo (A) Experimental timeline of CDX model and the fold change of tumor volume in CAR-T cell-treated CDX mouse model in the presence or absence of miltefosine administration (PBS group, n = 3; other groups, n = 5). (B) Tumor weight of each group at the end of experiment (PBS group, n = 3; other groups, n = 5). (C) The percentages of mesothelin- and hCD3-positive area in tumor tissues (PBS group, n = 2; other groups, n = 5). Scale bar: 200 μm. (D) The fold change of tumor volume in CDX mouse model treated with CAR-T cells derived from another donor, in the presence or absence of miltefosine or anti-PD-1 antibody treatment ( n = 4). (E) The proportion of hCD3 + T cells in the peripheral blood of CDX mouse model ( n = 4). (F) The fold change of tumor volume in CAR-T cell-treated PDX mouse model, in the presence or absence of miltefosine or anti-PD-1 antibody treatment ( n = 4). (G) The proportion of hCD3 + T cells in the peripheral blood of PDX mouse model ( n = 4). Unpaired t test was used in statistical analysis. (H) Experimental timeline of the OT-1-treated melanoma isograft tumor model. (I) The fold change in tumor volume in the OT-1-treated melanoma isograft tumor model in the presence or absence of miltefosine administration ( n = 7). Unpaired t test was used in statistical analysis. NS, not significant, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. All error bars denote SEM. See also Figure S7 .

Article Snippet: Rabbit anti- human CD3 Antibody (clone EP41) , ZSGB-BIO , Cat#ZA-0503.

Techniques: In Vivo, Derivative Assay

Journal: Cell Reports Medicine

Article Title: Miltefosine reinvigorates exhausted T cells by targeting their bioenergetic state

doi: 10.1016/j.xcrm.2024.101869

Figure Lengend Snippet:

Article Snippet: Rabbit anti- human CD3 Antibody (clone EP41) , ZSGB-BIO , Cat#ZA-0503.

Techniques: Functional Assay, Flow Cytometry, Control, Recombinant, Lysis, Activation Assay, Transfection, Luciferase, Viability Assay, SYBR Green Assay, RNA Sequencing Assay, Construct, Plasmid Preparation, Software

Journal: International Journal of Ophthalmology

Article Title: Association of TCR-signaling pathway with the development of lacrimal gland benign lymphoepithelial lesions

doi: 10.3980/j.issn.2222-3959.2015.04.08

Figure Lengend Snippet: Primers used for RT-PCR

Article Snippet: All control patients had normal circulating IgG4 ( ). table ft1 table-wrap mode="anchored" t5 caption a7 Groups Mean age (a) Gender Affected eyes IgG4 level M F L R Bilateral High Normal Experimental group 50 2 7 2 0 7 9 0 Control group 46.4 3 6 5 4 0 0 9 Open in a separate window caption a8 Baseline clinical characteristics of patients in the experimental and control groups Major reagents The following reagents were used: polymerase chain reaction (PCR) mix kit (Sangon Biotech, Shanghai, China); RNA reverse transcription kit (New England Biolabs, Ipswich, MA, USA); rabbit anti-human CD3 and rabbit anti-human CD4 antibodies (ZSGB-Bio, Beijing, China); mouse anti-human IL-10 antibody (Abcam, Cambridge, MA, USA); universal two-step immunohistochemistry kit, horse radish peroxidase (HRP)-conjugated goat anti-mouse IgG, and HRP-conjugated goat anti-rabbit IgG (ZSGB-Bio).

Techniques:

Journal: Experimental Animals

Article Title: NOD- Rag2 null IL-2Rγ null Mice: An Alternative to NOG Mice for Generation of Humanized Mice

doi: 10.1538/expanim.63.321

Figure Lengend Snippet: Percentages of T-cell subsets in the spleen, thymus, and peripheral blood of HSC-transplanted mice

Article Snippet: To identify human cells in the spleen of transplanted mice, the spleens were removed from the mice after blood removal, and tissues were fixed with 10 nM formalin (10 nM Mildform; Wako, Tokyo, Japan), embedded in paraffin, and stained with hematoxylin and eosin (HE), mouse anti-human CD45 antigen monoclonal antibody (clone 2B11+PD7/26; Dako Cytomation, Glostrup, Denmark), rabbit anti-human CD3 monoclonal antibody (clone SP7; Nichirei, Tokyo, Japan), and mouse anti-human CD79a monoclonal antibody (clone JCB117; Nichirei).

Techniques: Injection